维生素K-氯高铁血红素溶液
Vitamin K1
- Hemin Solution Intended Use Vitamin K1
- Hemin Solution is used as a culture medium
enrichment for anaerobic microorganisms. Summary and Explanation CDC Anaerobe 5% Blood Agar was developed at the Centers
for Disease Control and Prevention as a nonselective medium
for the isolation and cultivation of a wide variety of obligately
anaerobic microorganisms, particularly those found in
clinical materials. The medium is enriched with vitamin K1
and hemin. Principles of the Procedure Gibbons and MacDonald reported isolating strains of Bacteroides
melaninogenicus (Prevotella melaninogenica) that require
hemin and vitamin K1 for growth. Vitamin K1 enhances
the growth of some strains of Bacteroides and certain grampositive
nonsporeformers. The inclusion of Vitamin K1
- Hemin
in anaerobic culture media has been suggested by many
investigators.Formula BBL™ Vitamin K1
-Hemin Solution Approximate Formula* Per Liter Hemin......................................................................... 0.5 g Sodium Hydroxide....................................................... 0.4 g Phytomenadione (Vitamin K1
)...................................... 0.05 g Ethyl Alcohol, 95% ................................................... 10.0 mL *Adjusted and/or supplemented as required to meet performance criteria. Procedure Vitamin K1
-Hemin Solution is a ready-to-use solution. The
solution cannot be heated and must be added aseptically in the
proper amounts to media that have been autoclaved and cooled
to 50-55°C.Vitamin K1
-Hemin Solution is usually employed in prepared
media at a final concentration of 5% for optimal results. Add
Vitamin K1
-Hemin Solution as required. Expected Results Refer to appropriate references and procedures for results. User Quality Control Identity Specifications BBL™ Vitamin K1
-Hemin Solution Appearance: Dark blackish-brown, opaque. Cultural Response BBL™ Vitamin K1
-Hemin Solution Prepare medium with added Vitamin K1
-Hemin Solution. Inoculate and incubate
at 35-37°C for up to 4 days under appropriate atmospheric conditions. ORGANISM ATCC™ INOCULUM CFU recovery Bacteroides levii 29147 103
-104 Good Bacteroides vulgatus 8482 103
-104 Good References 1. Dowell, Lombard, Thompson and Armfield. 1977. Media for isolation, characterization and identification
of obligately anaerobic bacteria. CDC laboratory manual. CDC, Atlanta, Ga.2. Jousimies-Somer, Summanen and Finegold. 1999. In Murray, Baron, Pfaller, Tenover and Yolken
(ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C. 3. Gibbons and MacDonald. 1960. J. Bacteriol. 80:164. 4. Finegold, Sutter, Attebery and Rosenblatt. 1974. In Lennette, Spaulding and Truant (ed.), Manual of
clinical microbiology, 2nd ed. American Society for Microbiology, Washington, D.C. 5. Murray and Citron. 1991. In Balows, Hausler, Herrmann, Isenberg and Shadomy (ed.), Manual of
clinical microbiology, 5th ed. American Society for Microbiology, Washington, D.C.