MTT 细胞增殖和细胞毒性检测试剂盒 Q2483
订购信息货号规格价格Q2483-250 Tests 250 Tests ¥468.00Q2483-500 Tests500 Tests¥838.00Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines,
mitogens, and drugs. Tetrazolium salts (e.g. MTT, XTT, WST-1 and WST-8) are especially useful for assaying the
quantification of viable cells, because they are cleaved to form a formazan dye only by metabolic active cells.
MTT Cell Proliferation Assay Kit provides a convenient tool for studying the induction and inhibition of cell proliferation in
vitro. In this assay, MTT is taken up by cells due to its net positive charge and the plasma membrane potential. It is reduced
to formazan by intracellular NAD(P)H-oxidoreductases. This kit also allow investigators to screen drug candidates involved
in regulation of cell cycle.概述MTT细胞增殖检测试剂盒(MTT Cell Proliferation and Cytotoxicity Assay Kit)被广泛应用于细胞增殖和细胞毒性检测。MTT可以被线粒体内的一些脱氢酶还原生成结晶状的深紫色产物Formazan,在特定溶剂存在的情况下,可以被完全溶解,然后通过酶标仪可以测定570 nm波长附近的吸光度。应用细胞增殖与毒性检测组份Component A: MTT; Component B: MTT Solvent; Component C: Formazan Solubilization Solution250 Tests 500 Tests Component A: MTT Reagent12.5 mg X 125 mg X 1Component B: MTT Solvent 1.25 mL X 2 5 mL X 1 Component C: Formazan Solubilization Solution 27.5 mL X 1 27.5 mL X 2