| 种属 | 种属详情 | 细胞 | 适用的酶 | 适用的酶浓度 | 介质 | 参考文献 |
|---|
| Mouse | Mouse | 胰岛 | 胶原酶 Type 4 | 0.001 | HBSS | Low, L., Zavortink, M., Mitchell, J., Gan, W., Do, O., Schwiening, C., Gaisano, H. and Thorn, P.: Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature, Diabetologia 57, 1655, 2014 |
| Mouse | Mouse | 胰岛 | 胶原酶 | 0.1-0.25% | HBSS | Koh, D., Moody, M. and Jo, J.: Collection of Islets of Langerhans using an Equilibrium Method., Biotechniques 55, 34-7, 2013 |
| Mouse | Mouse, 8-12 week | 胰腺瀑样 | 胶原酶 | 0.00012 | DMEM | Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent |
| Mouse | Mouse, 8-12 week | 胰腺瀑样 | 中性蛋白酶 | 0.00012 | DMEM | Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent |
| Mouse | Mouse, embryonic | 胰腺祖细胞 | 中性蛋白酶 | 0.00125 | DMEM | Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M. and Grapin-Botton, A.: Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro., Development 140, 4452-62, 2013 |
| Mouse | Mouse | 胰岛 | 胶原酶 | 0.002 | RPMI 1640 | Bertera, S., Balamurugan, A., Bottino, R., He, J. and Trucco, M.: Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin., J Transplant 2012, 856386, 2012 |
| Mouse | Mouse | 胰岛 | 胶原酶 Type 4 | 0.002 | RPMI-1640 | Ding, G., Wang, F., Shu, J., Tian, S., Jiang, Y., Zhang, D., Wang, N., Luo, Q., Zhang, Y., Jin, F., Leung, P., Sheng, J. and Huang, H.: Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyper |
| Mouse | Mouse, 10 week | 胰岛 | 胶原酶 | 0.002 | RPMI 1640 | Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem C |
| Mouse | Mouse, 6-8 month | 胰岛 | 胶原酶 Type 4 | 0.001 | HBSS | Taguchi, Y., Tasaki, Y., Terakado, K., Kobayashi, K., Machida, T. and Kobayashi, T.:Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice., J Endocrinol 206, 195-204, 2010 |
| Mouse | Mouse, adult, male | 腺泡的 | CLSPA | see reference | DMEM | Ji, B., Gaiser, S., Chen, X., Ernst, S. and Logsdon, C.: Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation., J Biol Chem 284, 17488, 2009 |
| Mouse | Mouse, adult, male | 腺泡的 | 大豆胰蛋白酶抑制剂 | 1e-005 | DMEM | Ji, B., Gaiser, S., Chen, X., Ernst, S. and Logsdon, C.: Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation., J Biol Chem 284, 17488, 2009 |
| Mouse | Mouse, 3-4 week, 20-24 g | 胰岛 | 胶原酶 Type 4 | 0.002 | RPMI 1540 | Huang, H., Xie, Q., Kang, M., Zhang, B., Zhang, H., Chen, J., Zhai, C., Yang, D., Jiang, B. and Wu, Y.: Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonanc |
| Mouse | Mouse | 胰岛 | 胶原酶 | 1,000 u/ml | RPMI 1640 | Li, D., Yuan, Y., Tu, H., Liang, Q. and Dai, L.: A Protocol for Islet Isolation from Mouse Pancreas., Nat Protoc 4, 1649, 2009 |
| Mouse | Mouse | 胰岛 | 胶原酶 | 0.0014 | RPMI 1640 | Carter, J., Dula, S., Corbin, K., Wu, R. and Nunemaker, C.: A Practical Guide to Rodent Islet Isolation and Assessment., Biol Proced Online 11, 3, 2009 |
| Mouse | Mouse | 胰岛 | 胶原酶 | 0.03-0.08% | RPMI 1640 | Szot, G., Koudria, P. and Bluestone, J.: Murine Pancreatic Islet Isolation., J Vis Exp 7, 255, 2007 |
| Mouse | Mouse, 6-10 month | 胰腺胆管 | CLSPA | 50 u/ml | DMEM | Wang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J 25, 5049, 2006 |
| Mouse | Mouse, 6-10 month | 胰腺胆管 | 透明质酸酶 | 400 u/ml | DMEM | Wang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J 25, 5049, 2006 |
| Mouse | Mouse, 6-10 month | 胰腺胆管 | 大豆胰蛋白酶抑制剂 | 0.0002 | DMEM | Wang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J 25, 5049, 2006 |
| Mouse | Mouse, adult | 腺泡的 | CLSPA | 200 u/ml | see reference | Voronina, S., Barrow, S., Gerasimenko, O., Petersen, O. and Tepikin, A.: Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi., J Biol Chem 279, 27327 |
| Mouse | Mouse, 11-12 week | 胰岛 | 胶原酶 Type 4 | 0.002 | RPMI | Astrof, S., Crowley, D., George, E., Fukuda, T., Sekiguchi, K., Hanahan, D. and Hynes, R.: Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis., Mol Cell Biol 24, 8662-70, |
| Mouse | Mouse | 胰岛 | 胶原酶 Type 4 | 0.002 | HBSS | Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells, J |
| Mouse | Mouse | 胰岛 | 胶原酶 Type 4 | 0.002 | HBSS | Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping: DcR3/TR6 effectively prevents islet primary nonfunction after transplantation, Diabetes 52, 2279-86, 2003 |
| Mouse | Mouse, BALB/c, 6-8 week-old, either sex | 腺泡的 | 胶原酶 | 0.001 | Waymouth's MB | Kurup, S., and Bhonde, R.: Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells, JOP 3 (1), 8, 2002 |
| Mouse | Mouse | 胰岛 | 胶原酶 Type 2 | 0.002 | CF Medium | Koster, J., Marshall, B., Ensor, N., Corbett, J., and Nichols, C.: Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes, Cell 100, 645, 2000 |
| Mouse | Mouse | 胰岛 | 胶原酶 Type 4 | 0.004 | Gey's BSS | Strowski M, Parmar R, Blake A, Schaeffer J: Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice, Endocrinology 141, 111-7, 2000 |
| Mouse | Mouse | 腺泡的细胞和腺泡 | 胶原酶 Type 1 | see reference | see reference | Toivola, D., Ku, N., Ghori, N., Lowe, A., Michie, S. and Omary, M.: Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury., Exp Cell Res 255, 156, 2000 |
| Mouse | Mouse | 腺泡的细胞和腺泡 | CLSPA | see reference | see reference | Toivola, D., Ku, N., Ghori, N., Lowe, A., Michie, S. and Omary, M.: Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury., Exp Cell Res 255, 156, 2000 |
| Mouse | Mouse, albino, 25 g | 腺泡的 | CLSPA | see reference | see reference | Fogarty, K., Kidd, J., Tuft, R. and Thorn, P.: A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells., Biophys J 78, 2298, 2000 |
| Mouse | Mouse | 导管 | 木瓜蛋白酶 | 25 u/ml | DMEM /F-12 | Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:12.1, 1995 |
| Mouse | Mouse | 腺泡的 | 胶原酶 | 100 u/ml | HEPES | Jauch, P., Peterson, O., and Lauger, P.: Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells, J Membr Biol 94, 99, 1986 |
| Mouse | Mouse (C57BL/6J-ob/ob), 9-12 wks, male | 胰岛 | 透明质酸酶 | 0.005 | Kreb's Ringer bicarbonate buffer | Dalpe-Scott, M., Heick, H., and Begin-Heick, N.: Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release, Diabetes 32, 932, 1983 |
| Mouse | Mouse, male, 18-24 g | 腺泡的 | CLSPA | 70-90 u/ml | Krebs-Henseleit | Burnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini, J Biol Chem 257, 10523-8, 1982 |
| Mouse | Mouse, male, 18-24 g | 腺泡的 | 大豆胰蛋白酶抑制剂 | 0.0001 | Krebs-Henseleit | Burnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini, J Biol Chem 257, 10523-8, 1982 |
| Mouse | Mouse, 7-10 week | 胰岛 | 胶原酶 | see reference | DMEM | Yesil, P, Michel, M., Chwalek, K., Pedack, S., Jany, C., Ludwig, B., Bornstein, S. and Lammert, E.: A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas., Islets 1, 185, |
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