小鼠胰腺 worthington 组织分离指南
种属 种属详情细胞 适用的酶适用的酶浓度介质参考文献MouseMouse胰岛胶原酶 Type 40.001HBSSLow, L., Zavortink, M., Mitchell, J., Gan, W., Do, O., Schwiening, C., Gaisano, H. and Thorn, P.: Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature, Diabetologia 57, 1655, 2014MouseMouse胰岛胶原酶0.1-0.25%HBSSKoh, D., Moody, M. and Jo, J.: Collection of Islets of Langerhans using an Equilibrium Method., Biotechniques 55, 34-7, 2013MouseMouse, 8-12 week胰腺瀑样胶原酶0.00012DMEMHuch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-PotentMouseMouse, 8-12 week胰腺瀑样中性蛋白酶0.00012DMEMHuch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-PotentMouseMouse, embryonic胰腺祖细胞中性蛋白酶0.00125DMEMGreggio, C., De Franceschi, F., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M. and Grapin-Botton, A.: Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro., Development 140, 4452-62, 2013MouseMouse胰岛胶原酶0.002RPMI 1640Bertera, S., Balamurugan, A., Bottino, R., He, J. and Trucco, M.: Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin., J Transplant 2012, 856386, 2012MouseMouse胰岛胶原酶 Type 40.002RPMI-1640Ding, G., Wang, F., Shu, J., Tian, S., Jiang, Y., Zhang, D., Wang, N., Luo, Q., Zhang, Y., Jin, F., Leung, P., Sheng, J. and Huang, H.: Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine HyperMouseMouse, 10 week胰岛胶原酶0.002RPMI 1640Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem CMouseMouse, 6-8 month胰岛胶原酶 Type 40.001HBSSTaguchi, Y., Tasaki, Y., Terakado, K., Kobayashi, K., Machida, T. and Kobayashi, T.:Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice., J Endocrinol 206, 195-204, 2010MouseMouse, adult, male腺泡的CLSPAsee referenceDMEMJi, B., Gaiser, S., Chen, X., Ernst, S. and Logsdon, C.: Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation., J Biol Chem 284, 17488, 2009MouseMouse, adult, male腺泡的大豆胰蛋白酶抑制剂1e-005DMEMJi, B., Gaiser, S., Chen, X., Ernst, S. and Logsdon, C.: Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation., J Biol Chem 284, 17488, 2009MouseMouse, 3-4 week, 20-24 g胰岛胶原酶 Type 40.002RPMI 1540Huang, H., Xie, Q., Kang, M., Zhang, B., Zhang, H., Chen, J., Zhai, C., Yang, D., Jiang, B. and Wu, Y.: Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic ResonancMouseMouse胰岛胶原酶1,000 u/mlRPMI 1640Li, D., Yuan, Y., Tu, H., Liang, Q. and Dai, L.: A Protocol for Islet Isolation from Mouse Pancreas., Nat Protoc 4, 1649, 2009MouseMouse胰岛胶原酶0.0014RPMI 1640Carter, J., Dula, S., Corbin, K., Wu, R. and Nunemaker, C.: A Practical Guide to Rodent Islet Isolation and Assessment., Biol Proced Online 11, 3, 2009MouseMouse胰岛胶原酶0.03-0.08%RPMI 1640Szot, G., Koudria, P. and Bluestone, J.: Murine Pancreatic Islet Isolation., J Vis Exp 7, 255, 2007MouseMouse, 6-10 month胰腺胆管CLSPA50 u/mlDMEMWang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J 25, 5049, 2006MouseMouse, 6-10 month胰腺胆管透明质酸酶400 u/mlDMEMWang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J 25, 5049, 2006MouseMouse, 6-10 month胰腺胆管大豆胰蛋白酶抑制剂0.0002DMEMWang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis., EMBO J 25, 5049, 2006MouseMouse, adult腺泡的CLSPA200 u/mlsee referenceVoronina, S., Barrow, S., Gerasimenko, O., Petersen, O. and Tepikin, A.: Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi., J Biol Chem 279, 27327MouseMouse, 11-12 week胰岛胶原酶 Type 40.002RPMIAstrof, S., Crowley, D., George, E., Fukuda, T., Sekiguchi, K., Hanahan, D. and Hynes, R.: Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis., Mol Cell Biol 24, 8662-70,MouseMouse胰岛胶原酶 Type 40.002HBSSHaefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells, JMouseMouse胰岛胶原酶 Type 40.002HBSSWu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping: DcR3/TR6 effectively prevents islet primary nonfunction after transplantation, Diabetes 52, 2279-86, 2003MouseMouse, BALB/c, 6-8 week-old, either sex腺泡的胶原酶0.001Waymouth's MBKurup, S., and Bhonde, R.: Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells, JOP 3 (1), 8, 2002MouseMouse胰岛胶原酶 Type 20.002CF MediumKoster, J., Marshall, B., Ensor, N., Corbett, J., and Nichols, C.: Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes, Cell 100, 645, 2000MouseMouse胰岛胶原酶 Type 40.004Gey's BSSStrowski M, Parmar R, Blake A, Schaeffer J: Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice, Endocrinology 141, 111-7, 2000MouseMouse腺泡的细胞和腺泡胶原酶 Type 1see referencesee referenceToivola, D., Ku, N., Ghori, N., Lowe, A., Michie, S. and Omary, M.: Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury., Exp Cell Res 255, 156, 2000MouseMouse腺泡的细胞和腺泡CLSPAsee referencesee referenceToivola, D., Ku, N., Ghori, N., Lowe, A., Michie, S. and Omary, M.: Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury., Exp Cell Res 255, 156, 2000MouseMouse, albino, 25 g腺泡的CLSPAsee referencesee referenceFogarty, K., Kidd, J., Tuft, R. and Thorn, P.: A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells., Biophys J 78, 2298, 2000MouseMouse导管木瓜蛋白酶25 u/mlDMEM /F-12Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:12.1, 1995MouseMouse腺泡的胶原酶100 u/mlHEPESJauch, P., Peterson, O., and Lauger, P.: Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells, J Membr Biol 94, 99, 1986MouseMouse (C57BL/6J-ob/ob), 9-12 wks, male胰岛透明质酸酶0.005Kreb's Ringer bicarbonate bufferDalpe-Scott, M., Heick, H., and Begin-Heick, N.: Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release, Diabetes 32, 932, 1983MouseMouse, male, 18-24 g腺泡的CLSPA70-90 u/mlKrebs-HenseleitBurnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini, J Biol Chem 257, 10523-8, 1982MouseMouse, male, 18-24 g腺泡的大豆胰蛋白酶抑制剂0.0001Krebs-HenseleitBurnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini, J Biol Chem 257, 10523-8, 1982MouseMouse, 7-10 week胰岛胶原酶see referenceDMEMYesil, P, Michel, M., Chwalek, K., Pedack, S., Jany, C., Ludwig, B., Bornstein, S. and Lammert, E.: A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas., Islets 1, 185,更多信息,咨询上海睿铂赛生物。