Human soluble α-Klotho Assay Kit - IBL
名称:Human soluble α-Klotho Assay Kit - IBL
货号:27998-96Well
规格:EA说明书Details for:soluble α-Klotho ELISAA novel messenger of healthy bodies?!α-Klotho, named after the Greek Goddess of Fate, Clotho, who spins the thread of life, was originally characterized as an aging suppressor gene. Our ELISA is perfectly suited for the measurement of the product of this gene - the α-Klotho protein. α-Klotho is also known as the messenger of healthy bodies (Fantuzzi G Front Immunol. 2014 Jul 21;5:351).A defect in klotho gene expression in the mouse results in a syndrome that resembles human ageing, including a short lifespan, infertility, arteriosclerosis, skin atrophy, osteoporosis and emphysema. The absence of α-Klotho leads to a substantially lower bone density than usual (Kuro-o M et al. Nature. 1997 Nov 6;390(6655):45-51).Picture: α-Klotho deficiency leads to bone mass density loss as the comparison of wildtype (left) and α-Klotho knockout mice (right) demonstrates (picture kindly provided by Dr. Yoichi Nabeshima, Laboratory of Molecular Science, Institute of Biomedical Research and Innovation, Foundation for Biomedical Research and Innovation, Kobe, Japan)Since the discovery of α-Klotho, which was described in the publication mentioned above, several research areas have emerged which benefit from the determination of α-Klotho in terms of gaining valuable additional information:Bone metabolismAging processesNephrologyImmunology, etc.α-Klotho and FGF23 are the main mediators of calcium and phosphate homeostasis. Any kind of disturbance in this regard, such as a reduced or increased production of α-Klotho, leads to metabolic bone disorders (John GB et al. Am J Kidney Dis. 2011 Jul;58(1):127-34).It is already known that α-Klotho levels in blood decrease with age (Yamazaki Y et al. Biochem Biophys Res Commun. 2010 Jul 30;398(3):513-8). Low α-klotho levels are often associated with age-related diseases.The outcomes of research in the areas of bone metabolism and aging, as well as the fact that α-Klotho is produced exclusively in the kidneys and brain, have prompted investigations into the impact of changes in α-Klotho levels in renal disease. Studies have revealed that α-klotho levels are significantly decreased in the serum already at an early stage in chronic kidney disease (Pavik I et al. Nephrol Dial Transplant. 2013 Feb;28(2):352-9). Since chronic kidney disease frequently occurs secondary to other medical conditions, α-Klotho is receiving increasing attention as a marker of the occurrence of chronic kidney disease in primary diseases such as type 2 diabetes (Lee EY et al PLoS One. 2014 Aug 1;9(8):e102984), whereby the sample material should be noted (see image below).Figure 1: In type 2 diabetes patients with stable renal function, both plasma and urine α-Klotho levels are significantly increased compared to healthy controls. With decreasing renal function, shown as albuminuria, α-Klotho levels decrease in plasma while remaining stable in urine.Furthermore, α-Klotho is gaining increasing attention as a player in many immunological processes, which is reflected in the increase of publications on α-Klotho.Figure 2: The number of publications on α-Klotho has been steadily increasing since 2005.Separate control set available on requestLiterature referencesPoelzl G, Ghadge SK, Messner M, Haubner B, Wuertinger P, Griesmacher A, Doerler J, Ensinger C, Ulmer H, Zaruba MM.Klotho is upregulated in human cardiomyopathy independently of circulating Klotho levels.Sci Rep. 2018 May 30;8(1):8429. PubMed ID: 29849175Keywords: Serum, cardiomyopathy, heart failure, cardioprotection, Klotho upregulated locallyPako J, Bikov A, Barta I, Matsueda H, Puskas R, Galffy G, Kerpel-Fronius A, Antus B, Horvath I. Assessment of the circulating klotho protein in lung cancer patients. Pathol Oncol Res. 2018 Jun 12. PubMed ID: 29948618keywords: Plasma, lung cancer, mechanism of action, circulating levelsKuriyama N, Ozaki E, Mizuno T, Ihara M, Mizuno S, Koyama T, Matsui D, Watanabe I, Akazawa K, Takeda K, Takada A, Inaba M, Yamada S, Motoyama K, Takeshita W, Iwai K, Hashiguchi K, Kobayashi D, Kondo M, Tamura A, Yamada K, Nakagawa M, Watanabe Y.Association between α-Klotho and Deep White Matter Lesions in the Brain: A Pilot Case Control Study Using Brain MRI.J Alzheimers Dis. 2018;61(1):145-155.PubMed ID: 29154273keywords: blood alpha-klotho, cerebral DWML, useful clinical index of vascular cognitive impairmentMilovanova L, Fomin V, Moiseev S, Taranova M, Milovanov Y, Lysenko Kozlovskaya L, Kozlov V, Kozevnikova E, Milovanova S, Lebedeva M, Reshetnikov V.Effect of essential amino acid кetoanalogues and protein restriction diet on morphogenetic proteins (FGF-23 and Кlotho) in 3b-4 stages chronic кidney disease patients: a randomized pilot study.Clin Exp Nephrol. 2018 Jun 11. [Epub ahead of print]Pubmed ID: 29948444keywords: chronic kidney disease, CKD, serum alpha-klotho, cardiovascular calcification, cardiac remodelingSilva AP, Mendes F, Pereira L, Fragoso A, Gonçalves RB, Santos N, Rato F, Neves PL.Klotho levels: association with insulin resistance and albumin-to-creatinine ratio in type 2 diabetic patients. Int Urol Nephrol. 2017 Oct;49(10):1809-1814. PubMed ID: 28677090 key words: chronic kidney disease, diabetes, klothoDrew DA, Katz R, Kritchevsky S, Ix J, Shlipak M, Gutiérrez OM, Newman A, Hoofnagle A, Fried L, Semba RD, Sarnak M.Association between Soluble Klotho and Change in Kidney Function: The Health Aging and Body Composition Study. J Am Soc Nephrol. 2017 Jun;28(6):1859-1866. PubMed ID:28104822 keywords: serum, chronic kidney disease, CKD, kidney dysfunction, klotho, risk factorsTan SJ, Smith ER, Holt SG, Hewitson TD, Toussaint ND. Soluble klotho may be a marker of phosphate reabsorption.Clin Kidney J. 2017 Jun;10(3):397-404.PubMed ID:28616218 keywords: chronic kidney disease, CKD, phosphate reabsorption, soluble klotho, urinary phosphate excretion, serumPedersen L, Christensen LL, Pedersen SM, Andersen M.Reduction of calprotectin and phosphate during testosterone therapy in aging men: a randomized controlled trial.J Endocrinol Invest. 2017 May;40(5):529-538. PubMed ID: 28000180keywords: double-blinded study, plasma klotho, testosterone therapySeibert E, Radler D, Ulrich C, Hanika S, Fiedler R, Girndt M.Serum klotho levels in acute kidney injury.Clin Nephrol. 2017 Apr;87 (2017)(4):173-179.PubMed ID: 28157067 keywords: acute kidney injury, chronic kidney disease, CKD, significantly correlated to eGFR.Sze L, Neidert MC, Bernays RL, Zwimpfer C, Wiesli P, Haile SR, Brändle M, Schmid C. Gender dependence of serum soluble Klotho in acromegaly.Clin Endocrinol (Oxf). 2014 Jun;80(6):869-73.PubMed ID: 24330426keywords: klotho, serum, genderKit size :12 x 8Method: ELISAIncubation time 1 x 1h, 2 x 30minStandard range 93.75 - 2000 pg/mLSpecimen / Volumes 100 µl serum, EDTA-plasmaSubstrate / isotope TMB 450 nmRegulatory Status: For research use only