For each assay in a given order, we will test three types of controls to make sure the assay is consistent from run to run. The DMSO only reactions are used as full activity control. The EDTA only reactions are used as the no or zero activity control. In addition, we includes one standard control compound in 10-dose IC50 format for every target in every order, including single dose duplicate measurements. All control results are included in your report.
The raw data, % enzyme activity, and control compound IC50 values will be reported in Excel format for single dose duplicate assays. For IC50 orders, raw data, IC50 values and curve fitting will be delivered in Excel format.
Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.
碱反应缓冲液；20 mM Hepes（pH 7.5），10 mM MgCl2、1 mM EGTA，0.02％Brij35、0.02 mg / ml BSA，0.1 mM Na3VO4、2 mM DTT，1％DMSO