Ovation® RNA-Seq System V2
The Ovation® RNA-Seq System V2 provides a rapid and sensitive whole transcriptome RNA amplification process for preparing amplified cDNA from total RNA or poly(A) selected mRNA from 500 pg – 100 ng. This robust workflow, based on NuGEN’s proprietary Ribo-SPIAtechnology, yields several micrograms of cDNA ready for multiple downstream applications including RNA-Seq, qPCR and archival storage for future analyses. When combined with the Ovation® Ultralow Library System V2 or Ovation® Rapid Library System, a complete RNA-Seq library can be generated in under 7 hours. The product is particularly useful for detection of rare transcripts, viruses and unknown pathogens.Key Features:Sample types include total RNA from LCM tissue, fine needle aspirates, sorted cells, liquid biopsy, prokaryotes, viruses and other challenging sample types. Automation enabled on multiple platforms including Agilent Bravo, Beckman Biomek FXP, Perkin Elmer Sciclone, and Hamilton Star/Starlet. ApplicationsRNA SequencingqPCRSample archivingSpecifications SpecificationDescriptionCompatible PlatformsIllumina HiSeq, MiSeq, NextSeq, MiniSeqStarting MaterialTotal RNAInput Amounts500 pg – 100 ng