小鼠(C57BL / 6J雄性)(Mus musculus)BAC库 RP24-263I11
RPCI-24 BAC库已在我们的实验室中从单只雄性C57BL / 6J小鼠(从杰克逊实验室获得)衍生的组织中构建。分离脾和脑基因组DNA样品,并用MboI部分消化。将大小选择的MboI片段克隆到BamHI位点之间的pTARBAC1载体中。将连接产物转化入DH10B电感受态细胞(BRL Life Technologies)。该文库已被排列在384孔微量滴定皿中,还被网格化到22x22cm尼龙高密度滤膜上,用于通过探针杂交进行筛选。每个杂交膜代表超过18,000个不同的小鼠BAC克隆,一式两份。RPCI-24公(C57BL / 6J)鼠标BAC库SegmentsCloning VectorDNARestrictionenzymePlate NumbersTotal PlatesEmpty WellsEmpty Wells (%)1pTARBAC1SpleenMboI1-161, 168-17728834643.1BrainMboI162-167, 178-2882pTARBAC1BrainMboI289-31328839513.6SpleenMboI314-576Total Library57674153.35SegmentsNon-insert Clones (%)Non-insert clonesNon-Recombinant Clones (pUC)Recombinant ClonesAverage Insert SizeRedundancy(Genome size:3,000 Mb)14.346071102,521160 Kbp5.520.55330106,108150 Kbp5.3Total Library2.451401208,629155 Kbp10.8